Genotypic Frequency Analysis of Mx Gene as Candidate Marker for Disease Resistance of Six Genetic Groups of Philippine Native Chicken (Gallus gallus domesticus L.)
J.D A. Valdez, A.J. Salces

ABSTRACT

The study was conducted to analyze the genotypic and allelic frequencies of Mx gene of Philippine native chickens considering the following fixed effects: genetic group, sex, plumage color, and geographical location.  DNA was extracted from chicken blood using FTATM WhatmanTM purification protocol and PCR-RFLP mismatched primers was used to amplify Mx gene exon 13 resulting to 101 bp PCR product.  PCR products were visualized through agarose gel electrophoresis and then digested using Hpal restriction enzyme. Digested products was visualized through polyacrylamide gel electrophoresis to determine the genotype of each individual chicken.  The result of genotyping showed that Philippine native chickens has significantly higher frequency of resistant Allele (62%) compared to commercial chicken breed (45%) (P<0.05).  Moreover, chickens with black plumage color and those coming from Mindanao has significantly higher frequency of resistant genotype and allele compared to brow and white plumage colored chickens and those chickens coming from Luzon and Visayas (P<0.05) but no significant difference in frequencies between sex and genetic group of an individual chicken (P>0.05).  It could be concluded that genotyping at nucleotide position 1,892 at exon 13 of coding sequence of Mx gene confirms that the frequency of resistant genotype AA and allele A of Philippine native chicken is significantly higher than the susceptible genotype GG and allele G.

Keyword(s):

diseases, gene, genotypic frequency, marker

Commodity:

Poultry

Year:

2015

Discipline:

Breeding